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Cleavage specificity of neprosin. A, IceLogo visualization of neprosin preference toward substrate subsites P4–P4′, derived from HeLa lysate digestion (n = 3316 and 3275 unique cleavage sites). Differences are displayed as the amino acid occurrence in a certain position, normalized to the natural occurrence in H. sapiens. B, heat map visualization of neprosin's cleavage specificity toward subsites P4–P4′ normalized to the natural occurrence in H. sapiens.
