Fig. 4.

Size distribution of identified proteins and peptides after HeLa whole-cell lysate digestion. A, size distribution of identified protein groups in HeLa lysate digested with neprosin (solid red line), LysC (solid dark gray line), and trypsin (solid light gray line) plotted against the in silico calculated size distribution of protein groups in the human proteome (dotted black line). B, average mass of identified peptides (black bars) and all MS-triggered peptide ions (gray bars) after digestion of HeLa cell lysate with trypsin, LysC, and neprosin as indicated.