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. 2005 Jan 12;33(1):289–297. doi: 10.1093/nar/gki170

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© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact journals.permissions@oupjournals.org.

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Reconstitution of 3′-PG processing and subsequent gap filling in SCAN1 extracts by recombinant TDP1. The plasmid substrate was treated with 3 mg/ml SCAN1 whole-cell extract (subject 1635) and analyzed as in Figure 3. In some cases, 25 ng of recombinant TDP1 and/or 200 ng of XRCC4/DNA ligase IV complex (Trevigen) were added. Some samples contained 5 mM EDTA instead of 0.5 mM MgCl₂, as indicated.