Table 1.

Structure and abbreviation of DNA duplexes^a

DNA	Abbreviation
37mer hemimethylated duplexes
5′-GACTGGTACAGTATCAGGCGCTGACCCACAACATCCGTGACCATGTCATAGTCCGMGACTGGGTGTTGTAGGCT-5′	GCGC/GMGC
5′-GACTGGTACAGTATCAGGPGCTGACCCACAACATCCGTGACCATGTCATAGTCCGMGACTGGGTGTTGTAGGCT-5′	GPGC/GMGC
5′-GACTGGTACAGTATCAGGAGCTGACCCACAACATCCGTGACCATGTCATAGTCCGMGACTGGGTGTTGTAGGCT-5′	GAGC/GMGC
Labeled 35/34mer duplex
5′-biotin-ACTGGTACAGTATCAGGCGCTGACCCACAACATCT-JOEGACCATGTCATAGTCCGCGACTGGGTGTTGTAGA-5′	biotin-GCGC-JOE
24mer duplex for the H5-exchange assay
5′-TAATAATGCGCTAATAATAATAATATTATTACGCGATTATTATTATTA-5′	GCGC

^aHhaI recognition site is in boldface, nucleotide at the target position is underlined; M, 5-methylcytosine; P, 2-aminopurine; and JOE, 6-carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein.