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. 2005 Jan 13;33(1):325–336. doi: 10.1093/nar/gki177

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© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact journals.permissions@oupjournals.org.

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TSA alters nascent strand abundance at uncharacterized sites. Nascent DNA isolated from cells treated with 100 ng/ml TSA for 4 h (A and B) or 8 h (C) was amplified by PCR with different 10mer primers using equal starting amounts of DNA. Products were purified, run on 2% agarose gels and stained with EtBr (inverse image). Samples from independent PCRs are shown to confirm the reproducibility of the assay.