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. 2005 Jan 13;33(1):325–336. doi: 10.1093/nar/gki177

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© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact journals.permissions@oupjournals.org.

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Cells treated with TSA initiate synthesis from the late-firing β-globin origin earlier in S phase. Mimosine-synchronized cells were released into fresh medium or medium containing 100 ng/ml TSA for the indicated number of hours and loaded on alkaline gels for isolation of 1–2 kb nascent DNA. The nascent DNA was analyzed by Q-PCR in triplicate to determine the abundance of nascent strands at STS-myc2 and STS-BG3. The data are from two independent nascent DNA preparations and are normalized to STS-myc2.