Fig. 7.

Properties of LiSDP1. a Subcellular localization of LiSDP1-mVenus in N. tabacum pollen tubes. Following 6 h of pollen germination, cells were stained for LBs using Nile Red and fluorescence was documented by laser scanning microscopy. From left to right: Nile Red fluorescence, mVenus fluorescence, merged image. Scale bar = 10 μm. 8 out of 8 pollen tubes analyzed showed comparable results. b Changes in expression of LiSDP1 in response to varying nitrogen supply as determined by Illumina RNA sequencing. Samples from 2 L. incisa cultures were sequenced in 4 technical replicates each. The fold change of gene expression is given after 3 days of nitrogen starvation compared to nitrogen replete conditions and the significance of this change is given as the P value adjusted for multiple testing at a false discovery rate of 0.05. c Changes in expression of LiSDP1 in response to varying nitrogen supply as determined by qRT-PCR. Transcript levels were normalized to RIBOSOMAL PROTEIN S21 transcripts. Expression is shown relative to time point 0 and error bars represent the standard error of the mean for 3 batches cultivated in parallel. The dotted line indicates the onset of nitrogen repletion and total fatty acid (TFA) levels are shown for comparison. d Schematic representation of LiSDP1 features with numbers indicating amino acid positions. Black segments represent residues that are identical or similar to A. thaliana SDP1 and the full line illustrates the conserved patatin domain. It includes the conserved GXSXG motif as well as the catalytic residue D448