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. 2017 Mar 15;117(6):2282–2291. doi: 10.1152/jn.00936.2016

Fig. 2.

Fig. 2.

IL-1β represses KIF3B and NOSIP expression through miR-372 in hNSC. A: mRNA levels of KIF3B and NOSIP in hNSC treated with either PBS control or IL-1β. B: microRNA microarray analysis was performed in hNSC treated with either PBS control or IL-1β (in triplicate) to examine expression profiles of miRNAs predicted to target both KIF3B and NOSIP. Twenty-two miRs were found to be upregulated following IL-1β treatment. C: mRNA levels of KIF3B and NOSIP in hNSC transfected with either negative control miR (miR-NC) or miR-372 (miR-372). D: protein levels of both KIF3B and NOSIP were analyzed by Western blot in hNSC with miR-NC/miR-372 transfection or with PBS/IL-1β treatment. Numbers below the bands indicate quantification relative to miR-NC or PBS (normalized by actin loading control). E: miR-372 level was analyzed by mature miR assay in hNSC treated with either PBS control or IL-1β. Data are means ± SD from at least 3 independent experiments. *P < 0.05; **P < 0.01 compared with PBS or miR-NC control.