Table 3.
Summary of Hprt gene mutations of UV-C-exposed Rev1B/B-254 and wild type cells
| Target | Wild type | Rev1B/B | |
|---|---|---|---|
| Mutations at dipyrimidine sites | |||
| Single base pair substitutions | 45 | 20 | |
| Transitions | |||
| GC > AT | CCTa | 11 | 6 |
| TCCa | 10 | 6 | |
| CT | 2 | 1 | |
| CC | 3 | 4 | |
| TC | 3 | 3 | |
| AT > GC | CTTa | 2 | 0 |
| TC | 1 | 0 | |
| Transversions | |||
| GC > TA | TC | 1 | 0 |
| TCCa | 1 | 0 | |
| AT > CG | CTTa | 1 | 0 |
| AT > TA | TT | 10 | 0 |
| Double base pair substitutions | 5 | 6 | |
| Mutations at non-dipyrimidine sites | |||
| Single base pair substitutions | 0 | 3 | |
| Transversions | |||
| GC > TA | 0 | 1 | |
| AT > CG | 0 | 1 | |
| AT > TA | 0 | 1 | |
| Double base pair substitutions | 1 | 1 | |
| Othersb | 30 | 40 | |
aMutations present in tripyrimidine sequences do not allow to define the dimer site of the UV-C-induced lesion.
bMutants that either display a frameshift, lack one or more exons in the Hprt mRNA, express no Hprt mRNA or contain no mutation in the coding region.