. 2005 Jan 14;33(1):430–438. doi: 10.1093/nar/gki191

Table 2.

Comparisons of the base-pair steps at the place of insertion, the intercalating protein side chains and the DNA kink angle of minor groove binding protein–DNA complexes

Protein	Amino acid	Step	Kink angle (°)		Reference
Wt-Sac7d/Sso7d	V, M	CpG	62 (∼70)^a	β-sheet	(10,11)
		ApA	60 (∼70)^a
Sac7d mutants
V26A/M29A	A, A	GpA	29 (∼52)^a	β-sheet	This work
V26F/M29F	F, F	CpG	58 (∼68)^a		This work
V26A	A, M	CpG	55 (∼69)^a		This work
		ApA	54 (∼68)^a
M29A	V, A	CpG	55 (∼66)^a		This work
		ApA	49 (∼60)^a
M29F	V, F	CpG	70 (∼80)^a		This work
		ApA	58 (∼68)^a
aTBP	F, F	TpA	45	β-sheet	(15)
	F, F	ApG	45
yTBP, hTBP	F, F	TpA	45	β-sheet	(16,17)
	F, F	ApA	45
PurR	L, (L′)	CpG	45	hinge α-helix	(18)
LacI	L	CpG	45	(leucine levers)	(19)
IHF	P	ApA	59 (>160)^a	β-ribbon arms	(20)
HU	P	TpT	57 (∼105–140)^a		(21)
HMG group				L-shaped 3-helix
Specific
HMG1	F	Pt-GpG	61		(22)
SRY^b	I, (F)	ApA	65 (∼75)^a		(23)
LEF-1^b	M, (F)	ApA	52 (∼90)^a		(24)
POU/HMG/DNA (Oct1/Sox2/FGF4)	M, (F)	ApA	45 (∼90)^a		(25)
Non-specific
HMG-D	M	TpA	49		(26)
	A	ApT	21
	V, T	GpA	32 (∼111)^a
yNHP6A^b	M, (Y)	TpG	25		(28)
	F	TpT	20 (∼70)^a

^aOverall bending angle of DNA in parenthesis.

^bNMR structure.