Fig. 7.

Signaling pathways of TREM2 protection against cerebral injury. a TREM2, p-ERK1/2, ERK1/2, p-NF-κB and NF-κB protein expression in cultured primary microglial cells were determined by western blotting 12h after OGDR, with or without transfection TREM2 siRNA or control. e These protein expression also in mice (sham, MCAO7d, sham + TREM2 siRNA, MCAO7d + TREM2 siRNA) were determined by western blot. Densitometric analyses of TREM2 versus β-Tublin (b,f), p-ERK1/2 versus ERK1/2 (c,g) and p-NF-κB versus NF-κB (d,h) were show as mean ± SEM; n = 5; # P < 0.05 compared control (normal) cells or the sham (sham + TREM2 siRNA) groups; *P < 0.05 compared with OGDR 12h cells transfected with control siRNA or MCAO7d mice; ΔP < 0.05 compared sham transfected with TREM2 siRNA to sham mice