Table 2.
Primer sequences
| Genes | Probe and Primer | Sequence | GenBank Accession Number |
|---|---|---|---|
| Leptin | Forward | 5'-CCAAAACCCTCATCAAGACC-3' | NM_008493 |
| Reverse | 5'-GTCCAACTGTTGAAGAATGTCCC-3' | ||
| UCP2 | Forward | 5'-CCGCATTGGCCTCTACGACTCT-3' | NM_011671 |
| Reverse | 5'-CCCCGAAGGCAGAAGTGAAGTG-3' | ||
| Adiponectin | Forward | 5'-CCCAAGGGAACTTGTGCAGGTTGGATG-3' | NM_009605.4 |
| Reverse | 5'-GTTGGTATCATGGTAGAGAAGAAAGCC -3' | ||
| C/EBPα | Forward | 5'-TGGACAAGAACAGCAACGAGTAC-3' | NM_001287523.1 |
| Reverse | 5'-CGGTCATTGTCACTGGTCAACT-3' | ||
| C/EBPβ | Forward | 5'-AAGCTGAGCGACGAGTACAAGA-3' | NM_001287739.1 |
| Reverse | 5'-GTCAGCTCCAGCACCTTGTG-3' | ||
| SREBP1c | Forward | 5'-AGCCTGGCCATCTGTGAGAA-3' | XM_006532714.2 |
| Reverse | 5'-CAGACTGGTACGGGCCACAA-3' | ||
| AMPK α1 | Forward | 5'-AAGCCGACCCAATGACATCA-3' | XM_011245321.1 |
| Reverse | 5'-CTTCCTTCGTACACGCAAAT-3' | ||
| AMPK α2 | Forward | 5'-GATGATGAGGTGGTGGA-3' | NM_178143.2 |
| Reverse | 5'-GCCGAGGACAAAGTGC-3' | ||
| GAPDH | VIC | 5'-TGCATCCTGCACCACCAACTGCTTAG-3' |