Fig. 3.

The effects of SMCHD1 KD in non-DUX4 permissive 4qB cells on PCDHβ cluster expression. The expression level of SMCHD1 after SMCHD1 shRNA KD in comparison with control KD shRNAs targeting luciferase and GFP is shown by RT-qPCR analysis in primary (a) myoblast and (b) myotube cells. RNA expression analysis was then assessed for PCDHβ cluster isoform members in primary (c) myoblast and (d) myotube cells. Results represent qRT-PCR analysis of the indicated gene after normalization to the internal control gene GUS1. For each gene, the value of expression in the control shRNA samples was then arbitrarily set to 1. *P < 0.05, t test; n = 6 (two shRNA constructs for each target on three independent cell lines); error bars = SEM