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. 2017 Jan 4;28(6):1814–1825. doi: 10.1681/ASN.2016090935

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Copyright © 2017 by the American Society of Nephrology

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Figure 1. — NCC and Kir4.1 are co-expressed along the DCT. Kir4.1 localized at the basolateral membrane of cTAL, DCT, and connecting tubule/collecting duct in Kcnj10^fl/fl mice. Colocalization of Kir4.1 and NKCC2 (A). Kir4.1 (red) was localized in cTAL (NKCC2, green). Colocalization of Kir4.1 and NCC (B). Kir4.1 (red) colocalized in DCT (NCC, green). Colocalization of Kir4.1 and CB-D28k in Kcnj10^fl/fl (C). Kcnj10 (green) was localized in DCT2 and connecting tubule, both of which express calbindin D 28K (CB D28k, red). Original magnification, ×400.