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. 2017 Jan 4;28(6):1814–1825. doi: 10.1681/ASN.2016090935

Figure 5.

Figure 5.

KS deletion of Kir4.1 decreased abundances of total and phosphorylated form of NCC, NKCC2, and modulated WNK-SPAK kinases. Representative immunoblot showing abundance of total and NCC phosphorylated at threonine 53 (A); abundance of total and NKCC2 phosphorylated at threonine 96/101 (B); abundance of total and SPAK phosphorylated at Serine 373 (C); total WNK4 abundance (D); WNK1 abundance (E). Protein abundance was normalized using β-actin. (F) Quantification of immunoblot results. Bars are mean±SEM. * P<0.001, **P<0.001 versus Kcnj10fl/fl mice. (G) Representative immunostaining for NCC on Kcnj10fl/fl and KS-Kcnj10−/− mouse kidneys. Original magnification, ×100.