Figure 3.

Detection of novel biomarkers in mouse. Immunofluorescence (CK20) and in situ hybridization (Tacstd2, Lcn2) demonstrate specificity for iAKI. Note that CK20 (red) was expressed in proximal tubules (megalin=green) and in intercalated cells (IC) (inset: CK20=red; AQP2=white). Note that Tacstd2 was expressed in distal nephron segments. Lcn2 RNA was expressed by thick ascending limbs of Henle (TALH) as well as ICs of the collecting ducts in iAKI. Timp2 and Igfbp7 RNA are shown for comparison. Note the expression of these genes in scattered glomerular (G) and tubular cells.