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. Author manuscript; available in PMC: 2017 Nov 8.
Published in final edited form as: Biochemistry. 2016 Oct 26;55(44):6150–6161. doi: 10.1021/acs.biochem.6b00453

Figure 3. ThT-positive assemblies formed by [Aβ(M1-42)]DiY and [Aβ(M1-42)]Q-K remain in solution following centrifugation.

Figure 3

At the end of each ThT assay, six, 100 μl replicates from each concentration of a given peptide were pooled and the fluorescence measured (2× 100 μl replicated; Ex440, Em480) before (colored bars) and after (empty bars) centrifugation at 16,000 g for 30 minutes. Data displayed are the average of three separate experiments ± standard deviation. Signals before and after centrifugation were analyzed by Student t test and *** represents p < 0.0001.