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. Author manuscript; available in PMC: 2017 Jun 7.
Published in final edited form as: Peptides. 2015 Oct 31;75:8–17. doi: 10.1016/j.peptides.2015.10.007

Figure 4.

Figure 4

Ability of four chiral diazepine analogs (3F, 9D, 9F, 9G); a nonselective Bn analog agonist (Peptide #1); a nonpeptide agonist MK-5046; GRP and NMB to stimulate [3H]IP generation in cells containing human, rat or mouse BRS-3: (A) hBRS-3/BALB, (B) rBRS-3/BALB and (C) mBRS-3/BALB cells. [3H]IP was determined after a 60 minutes incubation at 37°C after loading the cells for 48 hours with 3 μCi/ml myo-[2-3H]inositol as described in Materials and Methods. The results are the mean and S.E.M. from at least three separate experiments and in each experiment the data points were determined in duplicated. With hBRS-3/BALB cells, the maximal stimulated [3H]IP value by 1 μM MK-5046 was 70,268 ± 4,912 dpm, and the control value was 18,251 ± 1,632 dpm (n=13). With rBRS-3/BALB cells, the maximal stimulation of a 1 μM concentration of MK-5046 was 21,812 ± 1,721 dpm, and the control value was 3,749 ± 315 dpm (n=7). With mBRS-3/BALB cells, the maximal stimulation of a 1 μM concentration of MK-5046 was 62,992 ± 9,298 dpm, and the control value was 16,160 ± 2,396 dpm (n=3). Abbreviations: See in table 1 and 2 legends.