Table 1. Multiplex Real-Time PCR Results (Ct Values) for gp130 and HPRT1 Expression.
The panel of TNBC cell lines express gp130 signal transduction protein. MDA-MB-231, HS578T Hs578T and MDA-MB-468 cells were tested for the presence of gp130 mRNA expression via QRT-PCR. THP-1 cells served as a positive control. Significant message was observed in all cell lines.
| Housekeeping Gene | Gene of Interest | |||
|---|---|---|---|---|
|
|
|
|||
| Replicate 1 | Replicate 2 | Replicate 1 | Replicate 2 | |
|
|
|
|||
| HPRT1 | HPRT1 | gp130 | gp130 | |
| MDA-MB-231 Sample 1 | 20.8542 | 21.0557 | 20.7459 | 21.0018 |
| MDA-MB-231 Sample 2 | 20.5117 | 20.8309 | 20.6094 | 20.9634 |
| MDA-MB-231 Sample 3 | 20.4699 | 20.648 | 20.8029 | 20.8053 |
| Hs578T Sample 1 | 21.6563 | 21.911 | 20.113 | 20.4201 |
| Hs578T Sample 2 | 21.477 | 21.7132 | 19.7947 | 19.9812 |
| Hs578T Sample 3 | 21.6563 | 21.8859 | 20.0868 | 20.5735 |
| MDA-MB-468 Sample 1 | 20.8628 | 21.2024 | 22.2648 | 22.6831 |
| MDA-MB-468 Sample 2 | 21.3967 | 21.6432 | 22.7584 | 22.9019 |
| MDA-MB-468 Sample 3 | 21.2865 | 21.6053 | 22.6902 | 22.9063 |
| THP-1 Sample 1 | 21.1378 | 21.2445 | 24.2374 | 24.2065 |
| THP-1 Sample 2 | 20.9466 | 21.2468 | 23.886 | 24.1176 |
| THP-1 Sample 3 | 21.0651 | 21.1598 | 24.0519 | 24.2497 |