Figure 3.

Accuracy and efficiency of the PurAmp protocol for wide-range copy number quantification. Xist real-time PCR plots generated from individual embryos or cells processed with the PurAmp method. In the samples analyzed, the number of Xist genomic DNA + cDNA (RNA) templates increased right-to-left from 3 (yellow) to 10,000 (blue), as detailed in the Results section. Yellow, 3-cell male embryo; green, 4-cell male embryo; light purple, single blastomere from a 4-cell female embryo; red, 4-cell female embryo; blue, female blastocyst. Male samples also produced an Sry signal (inset), confirming sex assignment based on the presence or absence of Xist expression.