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. Author manuscript; available in PMC: 2017 Jun 7.
Published in final edited form as: J Cancer Stem Cell Res. 2016 Nov 30;4:e1005. doi: 10.14343/JCSCR.2016.4e1005

Figure 1. Ovarian ascites fluid contains protein molecule/s that stimulates migration of ovarian cancer cells.

Figure 1

Panel A. Dose-dependent effect of human ovarian ascites fluid on the migration of A2780 cells. Panel B. Migration of A2780 cells across Transwell membranes to various human ovarian ascites samples (A1–A6). Experiment was performed twice with similar results. *p<0.05. Panels C. Migration of CAOV3 and OVCAR4 ovarian cancer cell lines to ovarian ascites fluid (A). Experiment was performed twice with similar results. *p<0.05. Panel D. Chemotaxis (ascites in lower chamber only) vs chemokinesis (ascites fluid in upper and lower chamber) analysis of response of A2780 cells to ascites. Experiment was performed twice with similar results. *p<0.05. Panel E. The migration of A2780 cells across Transwell membranes in response to 10% ovarian ascites fluid, 10% ovarian ascites fluid dialyzed over a 3.5-kDa-MWCO membrane or 10% charcoal stripped ovarian ascites fluid. Experiment was performed twice with similar results. *p<0.05. Panel F. The temperature-dependence of 10% ascites preincubation on A2780 cell migration. *p<0.05. Experiment was performed twice with similar results. *p<0.05. Panel G. The migration of A2780 cells in response to 10% ovarian ascites fluid (A) preincubated with proteinase K (PK). To confirm the complete inhibition of PK by PMSF throughout the chemotaxis, a rescue experiment was performed in which 10% fresh was added. Experiment was performed twice with similar results. *p<0.05. Panel H. The migration of A549 cells across Transwell membranes in response to different fractions obtained by centrifugation in Centricon filters with different MWCOs. Experiment was performed twice with similar results. *p<0.05.