Skip to main content
. Author manuscript; available in PMC: 2017 Jun 7.
Published in final edited form as: J Cancer Stem Cell Res. 2016 Nov 30;4:e1005. doi: 10.14343/JCSCR.2016.4e1005

Figure 2. The stimulatory effects of human ovarian ascites fluid might be quenched by fibrinogen which suggest that vitronectin is a main chemoatractan/chemokinetic factor present in ovarian ascites fluids.

Figure 2

Panel A. The inhibitory effect of fibrinogen (FG) on the migration of A2780 cells in response to 10% human ovarian ascites fluid. Panel B. Migration of A2780 cell line to 10% ascites (A) is inhibited by 10% plasma (P) but not by 10% plasma without fibrinogen (P-Fb). Experiment was performed twice with similar results. *p<0.05. Panel C. ELISA analysis of vitronectin concentration in 6 ovarian ascites fluids samples. Panel D. Migration of A2780, CAOV3 and OVCAR4 ovarian cancer cell lines to vitronectin. Experiment was performed at least twice with similar results. *p<0.05 Panel E. Chemotaxis (vitronectin in lower chamber only) vs chemokinesis (vitronectin in upper and lower chamber) analysis of response of A2780 cells to vitronectin. Experiment was performed twice with similar results. *p<0.05. Panel FB. The adhesion of A2780 cells to fibronectin after stimulation with plasma, ascites and vitronectin for 5 min. Experiment was performed twice with similar results. *p<0.05.