Figure 1.

Confirmation of microarray results by sequantitative RT-PCR analysis. Total RNA was isolated from primary embryo fibroblasts (MEFs), ras^V12/E1A MEFs and ras^V12/E1A MEF-induced tumours. In these three preparations, mRNA encoding arginase 1, bone morphogenetic protein 10, cathepsin S, insulin-like growth factor binding protein 4, interferon stimulated gene 12, serum deprivation response, thrombomodulin, adenylate kinase 1, connective tissue growth factor and S100 calcium binding protein A11 were amplified by RT-PCR as described in Material and Methods section.