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. 2017 May 1;174(2):776–787. doi: 10.1104/pp.16.01844

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Figure 6. — Reducing barley stomatal density lowers gs and enhances WUE. A, Under well-watered conditions, a significant decrease in rate of carbon assimilation was observed in both HvEPF1OE lines. Under water-restricted conditions, there was no difference in assimilation. B, gs was significant decreased in HvEPF1OE lines grown under well-watered conditions in comparison to controls. Under water-restricted conditions, there was no difference in gs. C, Under well-watered conditions, a significant improvement in iWUE was observed in the HvEPF1OE-2 line when compared to control plants. Under water-restricted conditions, there was no difference in iWUE. D, Carbon isotope discrimination revealed a significant improvement in WUE of the HvEPF1OE-2 barley line under well-watered conditions. Under water-restricted conditions, both HvEPF1OE lines displayed significantly improved WUE in comparison to controls. n = 5 plants, asterisk indicates significance to at least P < 0.05 (Dunnett’s tests after one-way ANOVA for each watering group). Error bars represent se.