Figure 3.

Arabidopsis hydathode pores are responsive to light and ABA but fail to close in response to flg22 peptide treatment. Box plot representations of aperture (µm) of stomata and hydathode pores. At least three independent experiments were performed except for 5 and 10 µm flg22 treatment on stomata (two and one independent experiments, respectively). Number of measured pores are indicated in gray. Statistical groups were determined using a nonparametric Kruskal-Wallis test (P < 0.001) and are indicated by different letters. A, Apertures were measured on epidermal peels after 30 min incubation in darkness. Peels were then preincubated for 30 min in darkness with 0 to 100 µm ABA followed by a 2-h light incubation prior to aperture measurements. B, Apertures were measured on epidermal peels after 30 min incubation in darkness. Peels were then preincubated for 2 h in light. Peels were further incubated for 2 h with 0 to 10 µm flg22 prior to measurements.