Table I. Content of Dols, Prens, and phytosterols in Arabidopsis hairy roots exposed to various stress factors.
Dol, Pren, and phytosterol content was estimated using HPLC/UV and gas chromatography-flame ionization detection (GC-FID); n = 3. Results significantly (P < 0.05) different from the control are indicated with asterisks. H2O2, Hydrogen peroxide; MV, methyl viologen; t-BHP, tert-butyl hydroperoxide.
| Stress | Dol Content | Fraction of Dominating Dols (Dol-14 to Dol-17) in Total Dol Mixture | Fraction of Prens (Pren-14 to Pren-17) in Total Pren Mixture | Phytosterol Content | |
|---|---|---|---|---|---|
| μg g−1 fresh wt | % | mg g−1 fresh wt | |||
| Osmotic sorbitol (m) | Control | 4.4 ± 0.6 | 67 ± 7 | 15 ± 1 | 2.1 ± 0.4 |
| 0.2 | 4.9 ± 0.6 | 76 ± 9 | 22 ± 2 | 1.9 ± 0.3 | |
| 0.3 | 6.4 ± 0.6* | 91 ± 8 | 36 ± 3 | 1.9 ± 0.5 | |
| 0.4 | 6.9 ± 0.4* | 93 ± 9 | 35 ± 3 | 1.9 ± 0.7 | |
| 0.5 | 6.5 ± 0.1* | 88 ± 5 | 38 ± 4 | 1.9 ± 0.6 | |
| Oxidative | Control | 4.4 ± 0.2 | 66 ± 3 | 14 ± 2 | 2.0 ± 0.6 |
| 1 mm H2O2 | 5.3 ± 0.3* | 81 ± 4 | 23 ± 2 | 1.9 ± 0.2 | |
| 0.3 mm t-BHP | 5.8 ± 0.6* | 88 ± 5 | 26 ± 2 | 5.2 ± 0.4* | |
| 1 μm MV | 4.0 ± 0.2 | 61 ± 7 | 12 ± 1 | 4.0 ± 0.6* | |
| CdCl2 (μm) | Control | 4.4 ± 0.5 | 67 ± 7 | 15 ± 2 | 2.0 ± 0.3 |
| 25 | 5.6 ± 0.3* | 86 ± 8 | 27 ± 2 | 2.3 ± 0.3 | |
| 50 | 6.6 ± 0.6* | 91 ± 4 | 28 ± 2 | 2.0 ± 0.3 | |