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. 2017 Apr 6;174(2):857–874. doi: 10.1104/pp.17.00036

Table I. Content of Dols, Prens, and phytosterols in Arabidopsis hairy roots exposed to various stress factors.

Dol, Pren, and phytosterol content was estimated using HPLC/UV and gas chromatography-flame ionization detection (GC-FID); n = 3. Results significantly (P < 0.05) different from the control are indicated with asterisks. H2O2, Hydrogen peroxide; MV, methyl viologen; t-BHP, tert-butyl hydroperoxide.

Stress Dol Content Fraction of Dominating Dols (Dol-14 to Dol-17) in Total Dol Mixture Fraction of Prens (Pren-14 to Pren-17) in Total Pren Mixture Phytosterol Content
μg g−1 fresh wt % mg g−1 fresh wt
Osmotic sorbitol (m) Control 4.4 ± 0.6 67 ± 7 15 ± 1 2.1 ± 0.4
0.2 4.9 ± 0.6 76 ± 9 22 ± 2 1.9 ± 0.3
0.3 6.4 ± 0.6* 91 ± 8 36 ± 3 1.9 ± 0.5
0.4 6.9 ± 0.4* 93 ± 9 35 ± 3 1.9 ± 0.7
0.5 6.5 ± 0.1* 88 ± 5 38 ± 4 1.9 ± 0.6
Oxidative Control 4.4 ± 0.2 66 ± 3 14 ± 2 2.0 ± 0.6
1 mm H2O2 5.3 ± 0.3* 81 ± 4 23 ± 2 1.9 ± 0.2
0.3 mm t-BHP 5.8 ± 0.6* 88 ± 5 26 ± 2 5.2 ± 0.4*
1 μm MV 4.0 ± 0.2 61 ± 7 12 ± 1 4.0 ± 0.6*
CdCl2m) Control 4.4 ± 0.5 67 ± 7 15 ± 2 2.0 ± 0.3
25 5.6 ± 0.3* 86 ± 8 27 ± 2 2.3 ± 0.3
50 6.6 ± 0.6* 91 ± 4 28 ± 2 2.0 ± 0.3