Figure 4.

PVX-based VIF induces no transgenerational flowering. A, RT-PCR detection of virally expressed Arabidopsis AtFT (FT) and PVX coat protein (CP) RNAs in seedlings grown from seeds collected from PVX/AtFT-infected tobacco (MM) and tomato plants. The positions and sizes of the 1 kb plus ladder marker are indicated. PVX-specific forward primer PP82 and gene-specific reverse primer PP356 were used for detection of the virally expressed AtFT (750 bp), TCPF and TCPR for PVX CP (714 bp), and EF1F and EF1R for the housekeeping gene NbEF1α (380 bp). Primer sequences are listed in Supplemental Table S1. The positive control was systemic leaf-tissue materials harvested from PVX/AtFT-infected MM tobacco. B, Tobacco plants grown from seeds harvested from PVX/AtFT-infected tobacco plants did not flower in LD. C, Tobacco grown from seeds collected from MM plants under SD remained in the vegetative phase in LD. Plants were photographed at 75 d after sowing seeds.