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. 2017 Apr 11;174(2):875–885. doi: 10.1104/pp.17.00392

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Figure 6. — Influence of polypeptide tags on AtFT florigenic activity. A, MM plants infected with PVX expressing AtFT with and without different tags flowered differently under noninductive LD conditions. Plants were photographed at 52 d postinoculation. B, Effect of expression of AtFT with and without different tags, on bolting of tobacco plants. The average stem length (cm) at 52 d postinoculation is shown. C, RT-PCR detection of virally expressed Arabidopsis AtFT, AtFT-His, His-AtFT, and AtFT-FLAG mRNAs in systemic leaf tissues of four different tobacco plants (lanes 1–4) infected with PVX/AtFT (AtFT), PVX/AtFT-His (AtFT-His), PVX/His-FT (His-FT), or PVX/AtFT-FLAG (AtFT-FLAG). PVX-specific forward primer PP82 and a gene-specific reverse primer (Supplemental Table S1) were used for RT-PCR detection. Positive controls (+) were the respective recombinant plasmids, while no DNA was used in the negative control PCR. The positions and sizes of 1 kb plus ladder are indicated.