Figure 7.

Viral transient expression of mono- and dicot FT genes promotes SD tobacco flowering under noninducing LD conditions. A to C, MM tobacco plants were mock-inoculated or inoculated with PVX/Hd3a or PVX/mHd3a. D to F, Tobacco plants were mock-inoculated or inoculated with PVX/SFT or PVX/mSFT. In three separate experiments, ectopic expression of Hd3a or SFT caused tobacco plants to bolt at 2to 3 WPI and flower at 5 WPI. Plants mock-inoculated or infected with PVX/mHd3a or PVX/mSFT did not flower. Plants were photographed at 5 WPI. Bar = 4 cm. G and H, RT-PCR detection and sequencing confirmation of virally expressed wild-type and mutant rice Hd3a and SFT mRNA in systemic leaves of infected tobacco plants. The wild type and mutated start codons are underlined.