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. 2017 Apr 5;174(2):943–955. doi: 10.1104/pp.17.00202

Figure 4.

Figure 4.

GC-MS analysis of in vitro assays with I. rubescens diTPS on a Cyclodex-β GC column. A, Extracted ion chromatograms of m/z 257 of in vitro assays with IrCPS coupled with different IrKSL. The characterized SmCPS5 (ent-CPP synthase from S. miltiorrhiza), SmCPS1 (normal-CPP synthase from S. miltiorrhiza), SmKSL1 (miltiradiene synthase from S. miltiorrhiza), AtKS (ent-kaurene synthase from Arabidopsis), and PcmISO1 (pimaradiene from P. banksiana) were used as positive controls, along with the corresponding authentic standard ent-atiserene. (1) Miltiradiene, (2) isopimaradiene, (3) ent-kaurene, (4) ent-atiserene, and (5) ent-isopimaradiene-like compound. Asterisk indicates the unexpected enzyme products identified in this study. B, Corresponding mass spectra of recombinant enzyme assay products and authentic standard. EIC, Extracted ion chromatograms.