Figure 1.

hsp101 knockout mutant is affected in polysome recovery. A to F, Polysome profiles on 7-d-old seedlings in the wild type (A–C) or hsp101 (D–F). A and D, Control 22°C. B and E, HS for 1 h at 37°C. C and F, Two hours of recovery at 22°C after HS. G, Percentage of polysomes for each condition. n = 3 biological repeats, mean ± sd. A t test was performed between wild type and hsp101 for control (P > 0.1), at 1 h 37°C (P > 0.1), and at 2 h recovery (P < 0.05).