spch-5 epidermal phenotype alleviated by BRs through protein stabilization. A, Representative images of some plants scored in B and C, with stomata marked in purple and clusters denoted by brackets. Bars = 50 µm. B, Stomatal index of abaxial epidermis of 23-d-old cotyledons from plants grown on control medium (with DMSO as mock treatment; gray) or with 50 nm
BL (purple). C, Clustering percentage in 23-d-old abaxial cotyledons of spch-5 under control medium (gray) or BL (purple). Error bars represent se. Asterisks indicate P < 0.05, and n.d indicates P > 0.05 (Student’s t test) compared to the control medium. D, Protein immunoprecipitation with anti-GFP antibody (top) in protein extracts from spch-3 plants transformed with SPCHpro:SPCH-5-GFP or SPCHpro: SPCHPPP-GFP. Antitubulin antibody used as loading control (bottom).