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. 2017 Mar 8;16(10):927–939. doi: 10.1080/15384101.2017.1295178

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 5. — 4E-BP1 effects on protein synthesis in the oocytes. (A) Renilla luciferase reporter carrying 5′UTR TOP motive of Eef2 co-injected with 4E-BP1-Wt or 4E-BP1-Ala RNA. In the control no 4E-BP1 RNA was used and the IRES motive Firefly Luciferase was used as a loading control. Chemiluminescence was measured in the post NEBD stage (mean value ± 6 and 4 %, Student's t-test, NS = non-significant) and MII stage oocytes (mean values ± SD, Student's t-test). Data are presented as mean ± SD, n ≥ 10 replicates. (B) Measurement of in situ translation intensity in the chromosomal area (CTA, mean value ± SD, Student's t-test, NS = non-significant) and perispindular translational area (PTA, mean value ± SD, Student's t-test) in the post NEBD oocytes, HPG (red) and DNA (blue). Data are presented as mean ± SD, n ≥ 21. Scale bar = 20 µm.