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. 2017 Apr 7;16(10):999–1007. doi: 10.1080/15384101.2017.1312237

Figure 1.

Figure 1.

Activation of Notch-1 in PC-3 cells. (A) Western blotting was used to detect the expression of Notch-1 in prostate cancer cells. PC-3 ICN: PC-3 with ICN plasmid transfection. (B) Immunofluorescence was performed in PC-3 cells with ICN transfection. (C) Western blotting analysis was conducted to measure the expression of Notch-1 targets in prostate cancer cells. (D) Real-time RT-PCR was performed to detect the mRNA levels of Notch-1 targets in prostate cancer cells. *P<0.05; **P<0.01 vs PC-3 cells.