Figure 3.
Down-regulation of Notch-1 retarded cell migration and invasion. (A) Western blotting was conducted to measure the efficacy of Notch-1 siRNA for knocking down Notch-1 in PC-3 cells. CS: control siRNA; NS: Notch-1 siRNA. (B) Cell detachment and attachment were measured in PC-3 cells transfected with Notch-1 siRNA. *P<0.05 vs PC-3 cells. (C) Left panel: Matrigel migration and invasion chamber assays were conducted to detect the invasive activity of PC-3 cells transfected with Notch-1 siRNA. Right panel: quantitative results are illustrated for left panel. *P<0.05 vs control.