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PC-3 ICN cells have the morphologic changes and EMT feature. (A) The PC-3 and PC-3 ICN cells were photographed under the microscope. (B) Western blotting was conducted to measure the protein levels of EMT markers in PC-3 ICN cells. (C) Real-time RT-PCR was performed to detect the mRNA levels of EMT markers in prostate cancer cells. *P<0.05; **P<0.01 vs PC-3 cells.
