(A) Study design. N = 12 per group, 48 rats in total. (B) %Freezing (mean ± SD) after each shock during the Training session in rats that received an intraperitoneal pre-training saline (SAL) or 0.1 mg/kg scopolamine (SCOP) injection, *significantly higher than after the first shock in SAL rats, #significantly higher than after the first shock in SCOP rats, §significantly different between SAL and SCOP rats (p ≤ .01). (C) %Freezing (median + interquartile range) during the 8-minute Test 1 session, *significantly lower than A SAL rats (p < .01). (D) %Freezing after shocks for A SAL and A SCOP rats. Colored circles indicate animals that were included in the subset of rats with postshock freezing scores close to the average of saline rats. (E) %Freezing (median + interquartile range) during the 8-minute Test 1 in the subset of six A SAL and five A SCOP rats with ‘average’ postshock freezing scores, *significantly lower than A SAL rats (p < .01). (F) %Freezing (median) during the 8-minute Test 1 and subsequent 16-minute Extinction phase. (G) %Freezing (median + interquartile range) during the 8-minute Test 2.