Table 1.
Summary of transfected cells
Growth Factor | G418 | FLAG | EGFP | Growth In | ||
Cell | Plasmid | Dependence | Sensitivity | Epitope | Expression | Simple Medium |
NRP-152 | none | x | x | - | - | - |
152-pIRES | pIRES-EGFP | x | - | - | x | - |
152-S3c | pIRES-S3c | - | - | x | x | x |
BPH-1 | none | n/a | x | - | - | n/a |
BPH-pIRES | pIRES-EGFP | n/a | - | - | x | n/a |
BPH-S3c | pIRES-S3c | n/a | - | x | x | n/a |
The table summarizes the cells used or made in the experiments described. Cells were transfected with the indicated plasmid, as described in Materials & Methods. G418 was added at 400 μg/ml. Growth factors were those added to 152 medium, but not to 154 medium, as described in Materials and Methods, for NRP-152 cells and transfectants.
X = presence of characteristic; --- = absence of characteristic.