Table 2. Differentially expressed genes of ESBL-producing E. coli following exposure to CORM-2 (250 μM) versus vehicle (2.5% DMSO).
| Gene | Fold change | Fold change | Gene product |
|---|---|---|---|
| symbol | First exposure | 20x pre-exposed | |
| CORM-2 vs | CORM-2 vs | ||
| first exposure | 20x pre-exposed | ||
| vehicle | vehicle | ||
| Represented in all enriched ontologies | |||
| polB | 22.5 | 25.6 | DNA polymerase II |
| sulA | 13.9 | 15.6 | suppressor of lon; inhibits cell division and ftsZ ring formation |
| recAa | 13.6 | 14.2 | DNA strand exchange and renaturation, DNA-dependent ATPase |
| yebG | 12.5 | 15.3 | DNA damage-inducible protein regulated by LexA |
| dinI | 11.8 | 17.6 | damage-inducible protein I |
| recN | 11.4 | 13.0 | protein used in recombination and DNA repair |
| umuD | 10.2 | 14.5 | SOS mutagenesis; error-prone repair |
| umuC | 9.1 | 10.3 | SOS mutagenesis and repair |
| lexA | 8.2 | 10.8 | regulator for SOS |
| ruvB | 3.8 | 3.8 | Holliday junction helicase subunit A |
| uvrD | 3.8 | 2.9 | DNA-dependent ATPase I and helicase II |
| ruvA | 2.5 | 3.3 | Holliday junction helicase subunit B |
| uvrA | 2.6 | 2.5 | excision nuclease subunit A |
| uvrB | 2.1 | 2.4 | DNA repair; excision nuclease subunit B |
| Represented only in cell communication, SOS response, cellular response to external stimulus, cellular response to extracellular stimulus, response to extracellular stimulus, DNA repair or cellular response to stress | |||
| ydjM | 21.3 | 18.2 | inner membrane protein regulated by LexA |
| dinB | 5.2 | 6.1 | damage-inducible protein P; putative tRNA synthetase |
| Represented only in cellular response to DNA damage stimulus | |||
| mutM | 25.2 | 25.8 | formamidopyrimidine/5-formyluracil/ 5-hydroxymethyluracil DNA glycosylase |
| recF | 7.5 | 5.7 | ssDNA and dsDNA binding, ATP binding |
| mug | 3.2 | 3.5 | G/U mismatch-specific DNA glycosylase |
| phr | -3.0 | -2.3 | deoxyribodipyrimidine photolyase |
| alkB | -2.9 | -2.6 | DNA repair system specific for alkylated DNA |
| Represented only in cellular response to DNA damage stimulus | |||
| ung | -2.6 | -2.0 | uracil-DNA-glycosylase |
| Represented only in response to extracellular stimulus | |||
| sspB | 2.9 | 2.4 | stringent starvation protein B |
| sspA | 2.3 | 2.0 | regulator of transcription; stringent starvation protein A |
| yjiY | -22.9 | -19.2 | putative carbon starvation protein |
| slp | -8.2 | -7.1 | outer membrane protein induced after carbon starvation |
| psiF | -3.3 | -2.3 | induced by phosphate starvation |
| rspB | -2.9 | -2.7 | starvation sensing protein |
Presented genes are derived from significant enrichment in the gene ontologies cell communication, SOS response, cellular response to external stimulus, cellular response to extracellular stimulus, response to extracellular stimulus, cellular response to DNA damage stimulus, DNA repair or cellular response to stress. n = 4
a also represented in virulence