Skip to main content
. 2017 Jun 7;12(6):e0178769. doi: 10.1371/journal.pone.0178769

Fig 1. Effects of Ang1-7 in vitro and in vivo.

Fig 1

Ang1-7 dose-response of leptin expression in AT (A, B) and isolated adipocytes (C, D). AT and isolated adipocytes were incubated with Ang1-7 for 24 h. Leptin mRNA expression (A, C) and secreted leptin (B, D) were measured as described in the Materials and Methods. Ang1-7 was administered intra-peritoneally over a 2-day period. Blood serum and peri-renal AT were collected 24 h later. Serum leptin levels (E) and leptin content in peri-renal AT (F) were measured as described in the Materials and Methods. Rat body weights (BW) at the time of blood sampling (G). Each column and bar represents the mean ± SEM of three separate experiments. An asterisk (*) indicates P<0.05 vs. vehicle. Secretion levels of leptin were normalized to total adipocyte protein, and expression of leptin mRNA was normalized to that of β-actin. AT leptin content was normalized to body weight.