Fig 2. Analysis of receptor types involved in high- and low-dose Ang1-7 regulation of leptin expression.

(A, C) AT and (E) isolated adipocytes were pre-treated with A779, PD123177, D-Pro⁷Ang1-7 (DA1-7), or candesartan (Cand) for 1 h prior to Ang1-7 addition. Control cells were treated with PBS for 24 h. The cells were incubated for 24 h prior to measuring leptin mRNA expression. (B, D) isolated adipocytes were pre-treated with A779 for 1 h prior to Ang1-7 addition, and incubated for 24 h prior to measuring leptin secretion. Secreted leptin was measured as described in the Materials and Methods of the supplemental data. Each column and bar represents the mean ± SEM of three separate experiments. An asterisk (*) indicates P<0.05 vs. vehicle. Leptin secretion levels were normalized to total adipocyte protein, and expression of leptin mRNA was normalized to that of β-actin.