Fig 4. Kif2a regulates the switch of NSCs/NPCs from proliferation to differentiation.

(A-B) Indicated plasmids (scramble shRNA, Kif2a shRNA1 or Kif2a shRNA1+Kif2a^Res) were introduced into E13.5 neocortex via in utero electroporation, and electroporated brain slices were co-immunostained for GFP (green) with Pax6 (red) or Tbr2 (red) at E15.5. Scale bars = 50 μm. (C-D) Quantification of the percentages of GFP⁺;Pax6⁺ or GFP⁺;Tbr2⁺ among total GFP⁺ cells. n = 300–500 cells from three different brains. *P < 0.05, **P < 0.01, ns = no significant difference; one-way ANOVA followed by Bonferroni post-hoc test. Data are presented as the mean ± SEM.