Fig. 1.

Oleic acid and elaidic acid remodel the pri-miR-7-1/protein complex (a) Molecular structure of OA and EA. (b) Schematic of the secondary structure of pri-miR-7 and CTL of miR-7 (in red) showing the experimentally derived model for pre-miR-7-1/HuR/MSI2 complex (Choudhury et al. 2013) [10]. (c) Electrophoretic mobility shift assay (EMSA) of pre-miR-7-1 in the presence of HeLa cell extracts to demonstrate the binding of proteins and to quantitate the effect of increasing concentrations of OA and EA treatment on the pre-miR-7-1/protein complex. The image is a representative of three independent experiments. Lanes 1 and 6 show EMSA with no extract. Lanes 2 and 7 show EMSA with HeLa extract only. Lanes 3, 4, and 5 represent EMSA with increasing concentrations of OA. Lanes 8, 9, and 10 represent EMSA with increasing concentrations of EA. (d) Densitometry analysis of EMSA results to quantitate the effect of OA and EA treatment. Mock control represents the pre-miR-7-1 incubated with HeLa cell extract only. OA and EA treatment values were normalized to values derived from mock treatment. The standard error of the mean (± SEM) was calculated using the replicates of three independent experiments. Student's t-test was performed to assess the statistical significance; *P < 0.05, **P < 0.01.