Fig. 2.

HuR and DHX9 protein are detected in the pre-miR-7/protein complex. The figure shows western blot-combined EMSA (WEMSA) of EMSA presented in Fig. 1c. (a) HuR and (b) DHX9 were detected using corresponding antibodies. Lanes 1 and 6 show WEMSA with no extract. Lanes 2 and 7 show WEMSA with HeLa extract only. Lanes 3, 4, and 5 represent WEMSA with increasing concentrations of OA. Lanes 8, 9, and 10 represent WEMSA with increasing concentrations of EA. All WEMSA blots were repeated for a minimum of three times.