Fig. 4.

High concentrations of oleic acid (OA) and elaidic acid (EA) affect the pre-miR-16/protein complex. (a) Electrophoretic mobility shift assay (EMSA) of pre-miR-16 in the presence of HeLa cell extracts and increasing concentrations of OA and EA treatment. The image is a representative of three independent experiments. Lanes 1 and 6 show EMSA with no extract. Lanes 2 and 7 show EMSA with HeLa extract only. Lanes 3, 4, and 5 represent EMSA with increasing concentrations of OA. Lanes 8, 9, and 10 represent EMSA with increasing concentrations of EA. (b) Densitometry analysis of EMSA results to quantitate the effect of OA and EA treatment. Mock control represents the pre-miR-16 incubated with HeLa cell extract only. OA and EA treatment values were normalized to values derived from mock treatment. The standard error of the mean (± SEM) was calculated using the replicates of three independent experiments. Student's t-test was performed to assess the statistical significance; **P < 0.01.