Table 2. Mutational analysis of the HBV polymerase and surface ORFs for the occult HBV subjects.
| Sample ID | Subgenotype | Mutations in Reverse Transcriptase (RT) | Anti-HBV Drug Resistant Mutations | Mutations in Surface (S) | Vaccine/Diagnostic Escape Mutations |
|---|---|---|---|---|---|
| O-EICIS.1 | D1 | rtV27F, rtN33D*, rtY54L | sT46F, sQ129R*# | sQ129R | |
| O-EICIS.3 | D1 | rtN33D*, rtW58G | sQ129R*#, sV177A# | sQ129R | |
| O-EICIS.5 | D1 | rtN33D*, rtY54F, rtR110G*# | sT46S, sQ129R*# | sQ129R | |
| O-EICIS.6 | D1 | rtN33D*, rtA200V* | sL98R, sQ129R*#, sL192F*# | sQ129R | |
| O-EICI.7 | D1 | rtN33D* | sQ129R*# | sQ129R | |
| O-EICI.11 | D1 | rtN53K#, rtY54L, rtM129T, rtA194V | sT45N, sT46S, sG112E#, sR122K*#, sG145A*#, sL186F | sR122K, sG145A | |
| O-EICI.18 | D1 | rtN33D*, rtL140P, rtI162T, rtQ215R# | sL84P, sQ129R*#, sS132P, sS154P#, sV177A#, sS207G | sQ129R | |
| O-EICIS.19 | D1 | rtN33D*, rtF46L, rtS105T | sL26P, sQ129R*# | sQ129R | |
| O-EICI.23 | D1 (8) | rtN33D*, rtL69P, rtL179P | sS61P#, sQ129R*#, sS171P | sQ129R | |
| D3 (2) | rtL91P#, rtH124N#, rtN131D | sF83L, sT125M#, sP127T#, sW172stop# | sW172stop | ||
| O-EICIS.26 | D1 | rtN33D* | sQ129R*# | ||
| O-EICI.27 | D1 | rtK32E*, rtN33D*, rtF183C, rtA211T | sQ129R*#, sL175V | sQ129R | |
| O-EICIS.13 | D2 | rtN53D#, rtY54H, rtV103I# | sP135H#, sG185E | ||
| O-EICIS.22 | D2 | rtN53D#, rtY54H, rtV103I#, rtE218A | sA128V#, sS210R | ||
| O-EICIS.15 | D3 | rtH124N*, rtN131D*, rtA186T, rtP196R | sL84P, sT125M*#, sP127T*#, sP188A | ||
| O-EICIS.9 | A1 | rtY126H#, rtL180M#, rtM204V# | rtL180M, rtM204V | sF134Y, sI195M# | |
| O-EICIS.10 | A1 | rtR110G#, rtY126H# | - | ||
| O-EICIS.20 | A1 | rtY126Q | sQ101H#, sT118K#, sP142L# |
Shown are the occult-associated mutations in the HBV reverse transcriptase and surface ORF. The S gene of the underlined patient IDs was cloned in order to evaluate their quasispecies diversity. All the dominant and clonal sequences were compared with the references of their respective subgenotypes. Mutations in bold indicate that their presence in the dominant viral strain.
* indicates that the mutations are present in more than one viral clone of the same patient.
# indicates that the particular mutation has been reported previously.