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. 2017 Jun 7;12(6):e0179038. doi: 10.1371/journal.pone.0179038

Fig 6. Overexpression of GhCOL1-A and GhCOL1-D rescued the late-flowering phenotype of the Arabidopsis co-2 mutant.

Fig 6

(A) Representative phenotype of 20 d Ler, co-2, 35S:GhCOL1-A and 35S:GhCOL1-D transgenic line in phytotron under LD conditions. Scale bar, 1 cm. (B) Flowering time was measured as the rosette leaves number per plant. Data represent a minimum of 10 plants for each line ± SE. (C) Detection of GhCOL1-A/D expression by qRT-PCR in 35S:GhCOL1s transgenic lines and co-2 under LD conditions. (D) The expression level of endogenous AtFT (AT1G65480) was determined by qRT-PCR. Data represent the mean ± SE (n = 3) obtained from three independent biological repeats in (C) and (D), and AtACT 2 (AT3G18780) was used as internal control. ** indicate significant differences compared with co-2 at P < 0.01 according to the Student’s t-test.