TABLE 3.
Medium (endpoint) | % of STs with germ cell differentiationa | % of STs with round spermatidsb | % of STs with elongated spermatidsb | % of STs with AKAP4-positive spermatidsb |
---|---|---|---|---|
KSR (45 days) | ||||
With constant rotation | 23.37 ± 3.01 (n = 4) | 72.16 ± 8.21 (n = 6) | 4.55 ± 2.61 (n = 6) | 0.00 ± 0.00 (n = 3) |
Without constant rotation | 35.70 ± 5.91 (n = 5) | 46.62 ± 13.01 (n = 6) | 11.89 ± 6.64 (n = 6) | 0.67 ± 0.67 (n = 6) |
Albumax (45 days) | ||||
With constant rotation | 69.45 ± 3.00 (n = 3) | 89.73 ± 4.91 (n = 6) | 46.16 ± 14.43 (n = 5) | 34.13 ± 23.02 (n = 2) |
Without constant rotation | 69.66 ± 6.25 (n = 9) | 68.66 ± 3.67 (n = 10) | 21.84 ± 3.54 (n = 10) | 10.65 ± 4.30 (n = 8) |
Values represent mean ± SEM; n equals the total number of testis fragments evaluated. The data without constant rotation was from Table 1.
Percentages are based on the total number of STs in whole testis fragment sections. Only slides containing an entire cross section of the testis fragments were used.
Percentages are based on the number of STs with germ cell differentiation.