TABLE 3.
Effects of Constant Rotation on Round, Elongated and AKAP4-Positive Spermatid Differentiation
| Medium (endpoint) | % of STs with germ cell differentiationa | % of STs with round spermatidsb | % of STs with elongated spermatidsb | % of STs with AKAP4-positive spermatidsb |
|---|---|---|---|---|
| KSR (45 days) | ||||
| With constant rotation | 23.37 ± 3.01 (n = 4) | 72.16 ± 8.21 (n = 6) | 4.55 ± 2.61 (n = 6) | 0.00 ± 0.00 (n = 3) |
| Without constant rotation | 35.70 ± 5.91 (n = 5) | 46.62 ± 13.01 (n = 6) | 11.89 ± 6.64 (n = 6) | 0.67 ± 0.67 (n = 6) |
| Albumax (45 days) | ||||
| With constant rotation | 69.45 ± 3.00 (n = 3) | 89.73 ± 4.91 (n = 6) | 46.16 ± 14.43 (n = 5) | 34.13 ± 23.02 (n = 2) |
| Without constant rotation | 69.66 ± 6.25 (n = 9) | 68.66 ± 3.67 (n = 10) | 21.84 ± 3.54 (n = 10) | 10.65 ± 4.30 (n = 8) |
Values represent mean ± SEM; n equals the total number of testis fragments evaluated. The data without constant rotation was from Table 1.
a
Percentages are based on the total number of STs in whole testis fragment sections. Only slides containing an entire cross section of the testis fragments were used.
b
Percentages are based on the number of STs with germ cell differentiation.