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. 2017 May 25;13(5):e1006411. doi: 10.1371/journal.ppat.1006411

Fig 7. Inhibition of the ZIKV protease activity, viral titer, protein expression, and viral RNA replication by NSC135618.

Fig 7

(A) SDS-page analysis of purified linked and unlinked NS2B/NS3 protease complex of ZIKV. ST, molecular weight (MW) standard. (B) Dose response inhibition of the unlinked ZIKV NS2B-NS3 protease by NSC135618. The NS2b/NS3 protease was at 150 nM. N = 3. (C) Dose-dependent inhibition of ZIKV by NSC135618 in A549 cells. Viral plaque reduction assay was used. N = 3. (D) Immunofluorescence assay (IFA) of inhibition of viral protein expression for ZIKV-infected A549 cells by NSC135618, using pan flavivirus anti-E antibody 4G2. (E) qRT-PCR analyses of inhibition of viral RNA from supernatant of ZIKV infected A549 cells by NSC135618. N = 3, ***, p<0.01, by one-way ANOVA. All dose response curves were fitted with the Sigmoidal model using the Origin6.0 software suite.